What Is Chemiluminescence Immunoassay | Immunoassay Analyzer Principle

release time:2021-08-19 11:55:21

1 What is chemiluminescence immunoassay?

Chemiluminescence immunoassay is an immunoassay instrument that directly labels antigen or antibody with chemiluminescent agent. Chemiluminescent immunoassay analyzer contains two parts, i.e. immuno-reaction system and chemiluminescent analysis system.

2 Chemiluminescence immunoassay analyzer principle

Chemiluminescence immunoassay analyzer uses chemiluminescent substances catalyzed by a catalyst and oxidant oxidation, the formation of an excited state intermediate.

When this excited state intermediate back to the stable ground state, while emitting photons (hM), the use of luminescence signal measurement instrument to measure the amount of light quantum yield.

The immunoreactive system is the direct labeling of luminescent substances (excited intermediates generated under reactant excitation) on antigens (chemiluminescent immunoassay) or antibodies (immunochemiluminescent analysis), or enzymes acting on luminescent substrates.

The core detector component in chemiluminescent immunoassay instruments is the photomultiplier tube (PMT), which is detected by a single photon and transmitted to an amplifier, and amplified by a high voltage current. The amplifier then converts the analog current into a digital current, which transmits the luminescence signal to a computer by an R232 data line and calculates it to produce clinical results.

Chemiluminescence immunoassay is an immunoassay method that uses chemiluminescent agents to directly label antigens or antibodies. Commonly used in the labeling of chemiluminescent substances are acridinium ester (AE). It is a very effective luminescent marker, its luminescence by the action of the initiating luminescent reagent, strong direct luminescence in one second, for rapid scintillation.

Prev Return Next

Biochemistry Analyzer: 11 Reasons Auto is Better than Semi-Automatic

2022-06-23

Biochemical analysis of the body blood samples forms the basis of medical diagnosis and plays a crucial role in treating various health ailments. Automated biochemical analyzers analyze body blood samples and evaluate the concentration of biochemical markers,

What are the 5 main tests of kidney function by biochemistry analyzer?

2021-12-30

Since the kidneys have such an important role for the human body, it is especially important to take care of their health. Because there are many diseases that are related to kidney. Therefore, a good kidney function test is an important tool to protect the kidneys. Biochemistry analyzer is a common equipment used to test kidney function, and there are mainly the following aspects about kidney function testing.

What is the use of POCT machine | POCT test machine

2021-09-17

The advent of POCT machines has made it possible to test and diagnose diseases quickly and easily. POCT machines also allow patients with chronic diseases to continuously monitor their status in their daily environment.